https://3demmethods.i2pc.es/index.php?action=history&feed=atom&title=2024Comet_TomoLive 2026-05-01T16:29:51Z Revision history for this page on the wiki MediaWiki 1.44.2 https://3demmethods.i2pc.es/index.php?title=2024Comet_TomoLive&diff=4639&oldid=prev 2024-08-08T06:10:30Z

<p>Created page with &quot;== Citation == Comet, Maxime / Dijkman, Patricia M. / Boer Iwema, Reint / Franke, Tilman / Masiulis, Simonas / Schampers, Ruud / Raschdorf, Oliver / Grollios, Fanis / Pryor, Edward E. / Drulyte, Ieva. Tomo Live: an on-the-fly reconstruction pipeline to judge data quality for cryo-electron tomography workflows. 2024. Biological Crystallography, Vol. 80, No. 4, p. 247-258 == Abstract == Data acquisition and processing for cryo-electron tomography can be a significant bo...&quot;</p> <p><b>New page</b></p><div>== Citation ==<br /> <br /> Comet, Maxime / Dijkman, Patricia M. / Boer Iwema, Reint / Franke, Tilman / Masiulis, Simonas / Schampers, Ruud / Raschdorf, Oliver / Grollios, Fanis / Pryor, Edward E. / Drulyte, Ieva. Tomo Live: an on-the-fly reconstruction pipeline to judge data quality for cryo-electron tomography workflows. 2024. Biological Crystallography, Vol. 80, No. 4, p. 247-258<br /> <br /> == Abstract ==<br /> <br /> Data acquisition and processing for cryo-electron tomography can be a significant<br /> bottleneck for users. To simplify and streamline the cryo-ET workflow,<br /> Tomo Live, an on-the-fly solution that automates the alignment and reconstruction<br /> of tilt-series data, enabling real-time data-quality assessment, has been<br /> developed. Through the integration of Tomo Live into the data-acquisition<br /> workflow for cryo-ET, motion correction is performed directly after each of the<br /> acquired tilt angles. Immediately after the tilt-series acquisition has completed,<br /> an unattended tilt-series alignment and reconstruction into a 3D volume is<br /> performed. The results are displayed in real time in a dedicated remote web<br /> platform that runs on the microscope hardware. Through this web platform,<br /> users can review the acquired data (aligned stack and 3D volume) and several<br /> quality metrics that are obtained during the alignment and reconstruction<br /> process. These quality metrics can be used for fast feedback for subsequent<br /> acquisitions to save time. Parameters such as Alignment Accuracy, Deleted Tilts<br /> and Tilt Axis Correction Angle are visualized as graphs and can be used as filters<br /> to export only the best tomograms (raw data, reconstruction and intermediate<br /> data) for further processing. Here, the Tomo Live algorithms and workflow are<br /> described and representative results on several biological samples are presented.<br /> The Tomo Live workflow is accessible to both expert and non-expert users,<br /> making it a valuable tool for the continued advancement of structural biology,<br /> cell biology and histology.<br /> <br /> == Keywords ==<br /> <br /> == Links ==<br /> <br /> https://journals.iucr.org/d/issues/2024/04/00/qv5004/<br /> <br /> == Related software ==<br /> <br /> == Related methods ==<br /> <br /> == Comments ==</div>

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