https://3demmethods.i2pc.es/index.php?action=history&feed=atom&title=2024Gillman_Cone 2026-06-13T12:18:03Z Revision history for this page on the wiki MediaWiki 1.44.2 https://3demmethods.i2pc.es/index.php?title=2024Gillman_Cone&diff=4664&oldid=prev 2024-08-12T06:11:46Z

<p>Created page with &quot;== Citation == Gillman, Cody / Bu, Guanhong / Danelius, Emma / Hattne, Johan / Nannenga, Brent L. / Gonen, Tamir. Eliminating the missing cone challenge through innovative approaches. 2024. Journal of Structural Biology: X, Vol. 9, p. 100102 == Abstract == Microcrystal electron diffraction (MicroED) has emerged as a powerful technique for unraveling molecular structures from microcrystals too small for X-ray diffraction. However, a significant hurdle arises with plat...&quot;</p> <p><b>New page</b></p><div>== Citation ==<br /> <br /> Gillman, Cody / Bu, Guanhong / Danelius, Emma / Hattne, Johan / Nannenga, Brent L. / Gonen, Tamir. Eliminating the missing cone challenge through innovative approaches. 2024. <br /> Journal of Structural Biology: X, Vol. 9, p. 100102<br /> <br /> == Abstract ==<br /> <br /> Microcrystal electron diffraction (MicroED) has emerged as a powerful technique for unraveling molecular<br /> structures from microcrystals too small for X-ray diffraction. However, a significant hurdle arises with plate-like<br /> crystals that consistently orient themselves flat on the electron microscopy grid. If the normal of the plate<br /> correlates with the axes of the crystal lattice, the crystal orientations accessible for measurement are restricted<br /> because the crystal cannot be arbitrarily rotated. This limits the information that can be acquired, resulting in a<br /> missing cone of information. We recently introduced a novel crystallization strategy called suspended drop<br /> crystallization and proposed that crystals in a suspended drop could effectively address the challenge of preferred<br /> crystal orientation. Here we demonstrate the success of the suspended drop approach in eliminating the missing<br /> cone in two samples that crystallize as thin plates: bovine liver catalase and the SARS‑CoV‑2 main protease<br /> (Mpro). This innovative solution proves indispensable for crystals exhibiting systematic preferred orientations,<br /> unlocking new possibilities for structure determination by MicroED.<br /> <br /> == Keywords ==<br /> <br /> == Links ==<br /> <br /> https://www.sciencedirect.com/science/article/pii/S2590152424000072<br /> <br /> == Related software ==<br /> <br /> == Related methods ==<br /> <br /> == Comments ==</div>

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