WikiSysop: Created page with "== Citation == I. Harley, F. Mazzotta, X. Shaulli, F. Scheffold, K. Landfester, and I. Lieberwirth, “Practical considerations for plunge freezing samples over 40° C for Cryo-EM,” Micron, p. 103745, 2024. == Abstract == Cryo-EM is now an established tool for examining samples in their native, hydrated states—a leap made possible by vitrification. Utilising this sample preparation method to directly visualise temperature-responsive samples allows for deeper insig..."

2024-12-12T06:34:16Z

Created page with "== Citation == I. Harley, F. Mazzotta, X. Shaulli, F. Scheffold, K. Landfester, and I. Lieberwirth, “Practical considerations for plunge freezing samples over 40° C for Cryo-EM,” Micron, p. 103745, 2024. == Abstract == Cryo-EM is now an established tool for examining samples in their native, hydrated states—a leap made possible by vitrification. Utilising this sample preparation method to directly visualise temperature-responsive samples allows for deeper insig..."

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== Citation ==

I. Harley, F. Mazzotta, X. Shaulli, F. Scheffold, K. Landfester, and I. Lieberwirth, “Practical considerations for plunge freezing samples over 40° C for Cryo-EM,” Micron, p. 103745, 2024.

== Abstract ==

Cryo-EM is now an established tool for examining samples in their native, hydrated states—a leap made possible
by vitrification. Utilising this sample preparation method to directly visualise temperature-responsive samples
allows for deeper insights into their structural behaviours under functional conditions. This requires samples to
be plunge-frozen at elevated temperatures and presents additional challenges, including condensation within the
blotting chamber and difficulties in maintaining a stable sample temperatures. Here, we address these challenges
and suggest practical strategies to minimise condensation and reduce temperature fluctuations during the
plunge-freezing of samples at elevated temperatures (>40 ◦C). By preheating equipment and reducing chamber
humidity and blotting times, we can improve sample preservation and grid reproducibility. These considerations
are then demonstrated on poly(N-isopropylacrylamide) microgels, which exhibit a volume phase transition due
to temperature changes.

== Keywords ==

== Links ==

https://www.sciencedirect.com/science/article/pii/S0968432824001628

== Related software ==

== Related methods ==

== Comments ==

2024Harley 40 - Revision history