https://3demmethods.i2pc.es/index.php?action=history&feed=atom&title=2024Henderikx_Vitrojet 2026-06-13T12:18:53Z Revision history for this page on the wiki MediaWiki 1.44.2 https://3demmethods.i2pc.es/index.php?title=2024Henderikx_Vitrojet&diff=4644&oldid=prev 2024-08-08T09:49:30Z

<p></p> <table style="background-color: #fff; color: #202122;" data-mw="interface"> <col class="diff-marker" /> <col class="diff-content" /> <col class="diff-marker" /> <col class="diff-content" /> <tr class="diff-title" lang="en"> <td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">← Older revision</td> <td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 09:49, 8 August 2024</td> </tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l28">Line 28:</td> <td colspan="2" class="diff-lineno">Line 28:</td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Links ==</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Links ==</div></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>https://<del style="font-weight: bold; text-decoration: none;">academic</del>.<del style="font-weight: bold; text-decoration: none;">oup</del>.<del style="font-weight: bold; text-decoration: none;">com</del>/<del style="font-weight: bold; text-decoration: none;">bib</del>/<del style="font-weight: bold; text-decoration: none;">article</del>/<del style="font-weight: bold; text-decoration: none;">25</del>/<del style="font-weight: bold; text-decoration: none;">3</del>/<del style="font-weight: bold; text-decoration: none;">bbae137</del>/<del style="font-weight: bold; text-decoration: none;">7638272</del></div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>https://<ins style="font-weight: bold; text-decoration: none;">journals</ins>.<ins style="font-weight: bold; text-decoration: none;">iucr</ins>.<ins style="font-weight: bold; text-decoration: none;">org</ins>/<ins style="font-weight: bold; text-decoration: none;">d</ins>/<ins style="font-weight: bold; text-decoration: none;">issues</ins>/<ins style="font-weight: bold; text-decoration: none;">2024</ins>/<ins style="font-weight: bold; text-decoration: none;">04</ins>/<ins style="font-weight: bold; text-decoration: none;">00</ins>/<ins style="font-weight: bold; text-decoration: none;">ih5005</ins></div></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Related software ==</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Related software ==</div></td></tr> </table>

WikiSysop https://3demmethods.i2pc.es/index.php?title=2024Henderikx_Vitrojet&diff=4637&oldid=prev 2024-08-08T06:06:11Z

<p>Created page with &quot;== Citation == Henderikx, Rene J. M. / Mann, Daniel / Domanska, Aušra / Dong, Jing / Shahzad, Saba / Lak, Behnam / Filopoulou, Aikaterini / Ludig, Damian / Grininger, Martin / Momoh, Jeffrey / others. VitroJet: new features and case studies. 2024. Biological Crystallography, Vol. 80, No. 4, p. 232-246 == Abstract == Single-particle cryo-electron microscopy has become a widely adopted method in structural biology due to many recent technological advances in microscope...&quot;</p> <p><b>New page</b></p><div>== Citation ==<br /> <br /> Henderikx, Rene J. M. / Mann, Daniel / Domanska, Aušra / Dong, Jing / Shahzad, Saba / Lak, Behnam / Filopoulou, Aikaterini / Ludig, Damian / Grininger, Martin / Momoh, Jeffrey / others. VitroJet: new features and case studies. 2024. Biological Crystallography, Vol. 80, No. 4, p. 232-246<br /> <br /> == Abstract ==<br /> <br /> Single-particle cryo-electron microscopy has become a widely adopted method<br /> in structural biology due to many recent technological advances in microscopes,<br /> detectors and image processing. Before being able to inspect a biological sample<br /> in an electron microscope, it needs to be deposited in a thin layer on a grid and<br /> rapidly frozen. The VitroJet was designed with this aim, as well as avoiding the<br /> delicate manual handling and transfer steps that occur during the conventional<br /> grid-preparation process. Since its creation, numerous technical developments<br /> have resulted in a device that is now widely utilized in multiple laboratories<br /> worldwide. It features plasma treatment, low-volume sample deposition through<br /> pin printing, optical ice-thickness measurement and cryofixation of pre-clipped<br /> Autogrids through jet vitrification. This paper presents recent technical<br /> improvements to the VitroJet and the benefits that it brings to the cryo-EM<br /> workflow. A wide variety of applications are shown: membrane proteins,<br /> nucleosomes, fatty-acid synthase, Tobacco mosaic virus, lipid nanoparticles, tickborne<br /> encephalitis viruses and bacteriophages. These case studies illustrate the<br /> advancement of the VitroJet into an instrument that enables accurate control<br /> and reproducibility, demonstrating its suitability for time-efficient cryo-EM<br /> structure determination.<br /> <br /> == Keywords ==<br /> <br /> == Links ==<br /> <br /> https://academic.oup.com/bib/article/25/3/bbae137/7638272<br /> <br /> == Related software ==<br /> <br /> == Related methods ==<br /> <br /> == Comments ==</div>

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