WikiSysop: Created page with "== Citation == R. Karimi, C. E. Coupland, and J. L. Rubinstein, “Vesicle Picker: A tool for efficient identification of membrane protein complexes in vesicles,” J. Structural Biology, vol. 216, no. 4, p. 108148, 2024. == Abstract == Electron cryomicroscopy (cryo-EM) has recently allowed determination of near-atomic resolution structures of membrane proteins and protein complexes embedded in lipid vesicles. However, particle selection from electron micrographs of t..."

2024-12-12T08:40:23Z

Created page with "== Citation == R. Karimi, C. E. Coupland, and J. L. Rubinstein, “Vesicle Picker: A tool for efficient identification of membrane protein complexes in vesicles,” J. Structural Biology, vol. 216, no. 4, p. 108148, 2024. == Abstract == Electron cryomicroscopy (cryo-EM) has recently allowed determination of near-atomic resolution structures of membrane proteins and protein complexes embedded in lipid vesicles. However, particle selection from electron micrographs of t..."

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== Citation ==

R. Karimi, C. E. Coupland, and J. L. Rubinstein, “Vesicle Picker: A tool for efficient identification of membrane protein complexes in vesicles,” J. Structural Biology, vol. 216, no. 4, p. 108148, 2024.

== Abstract ==

Electron cryomicroscopy (cryo-EM) has recently allowed determination of near-atomic resolution structures of
membrane proteins and protein complexes embedded in lipid vesicles. However, particle selection from electron
micrographs of these vesicles can be challenging due to the strong signal contributed from the lipid bilayer. This
challenge often requires iterative and laborious particle selection workflows to generate a dataset of high-quality
particle images for subsequent analysis. Here we present Vesicle Picker, an open-source program built on the
Segment Anything model. Vesicle Picker enables automatic identification of vesicles in cryo-EM micrographs
with high recall and precision. It then exhaustively selects all potential particle locations, either at the perimeter
or uniformly over the surface of the projection of the vesicle. The program is designed to interface with cryoSPARC,
which performs both upstream micrograph processing and downstream single particle image analysis.
We demonstrate Vesicle Picker’s utility by determining a high-resolution map of the vacuolar-type ATPase from
micrographs of native synaptic vesicles (SVs) and identifying an additional protein or protein complex in the SV
membrane.

== Keywords ==

== Links ==

https://www.sciencedirect.com/science/article/pii/S1047847724000881

== Related software ==

== Related methods ==

== Comments ==

2024Karimi Vesicle - Revision history