https://3demmethods.i2pc.es/index.php?action=history&feed=atom&title=2024Li_Subtraction2024Li Subtraction - Revision history2026-06-13T12:13:24ZRevision history for this page on the wikiMediaWiki 1.44.2https://3demmethods.i2pc.es/index.php?title=2024Li_Subtraction&diff=4793&oldid=prevWikiSysop: Created page with "== Citation == S. Li, M. Li, Y. Wang, and X. Li, “Subtraction of liposome signals in cryo-EM structural determination of protein-liposome complexes,” Chinese Physics B, 2024. == Abstract == Reconstituting membrane proteins in liposomes and determining their structure is a common method for determining membrane protein structures using single-particle cryo-electron microscopy (cryo-EM). However, the strong signal of liposomes under cryo-EM imaging conditions often..."2024-11-08T09:37:46Z<p>Created page with "== Citation == S. Li, M. Li, Y. Wang, and X. Li, “Subtraction of liposome signals in cryo-EM structural determination of protein-liposome complexes,” Chinese Physics B, 2024. == Abstract == Reconstituting membrane proteins in liposomes and determining their structure is a common method for determining membrane protein structures using single-particle cryo-electron microscopy (cryo-EM). However, the strong signal of liposomes under cryo-EM imaging conditions often..."</p>
<p><b>New page</b></p><div>== Citation ==<br />
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S. Li, M. Li, Y. Wang, and X. Li, “Subtraction of liposome signals in cryo-EM structural determination of protein-liposome complexes,” Chinese Physics B, 2024.<br />
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== Abstract ==<br />
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Reconstituting membrane proteins in liposomes and determining their structure is a common method for determining<br />
membrane protein structures using single-particle cryo-electron microscopy (cryo-EM). However, the strong signal of liposomes<br />
under cryo-EM imaging conditions often interferes with the structural determination of the embedded membrane<br />
proteins. Here, we propose a liposome signal subtraction method based on single-particle two-dimensional (2D) classification<br />
average images, aimed at enhancing the reconstruction resolution of membrane proteins. We analyzed the signal distribution<br />
characteristics of liposomes and proteins within the 2D classification average images of protein–liposome complexes<br />
in the frequency domain. Based on this analysis, we designed a method to subtract the liposome signals from the original<br />
particle images. After the subtraction, the accuracy of single-particle three-dimensional (3D) alignment was improved,<br />
enhancing the resolution of the final 3D reconstruction. We demonstrated this method using a PIEZO1-proteoliposome<br />
dataset by improving the resolution of the PIEZO1 protein.<br />
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== Keywords ==<br />
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== Links ==<br />
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https://iopscience.iop.org/article/10.1088/1674-1056/ad4cdb/meta<br />
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== Related software ==<br />
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== Related methods ==<br />
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== Comments ==</div>WikiSysop