https://3demmethods.i2pc.es/index.php?action=history&feed=atom&title=2024Liu_Graphene2024Liu Graphene - Revision history2026-05-24T21:06:42ZRevision history for this page on the wikiMediaWiki 1.44.2https://3demmethods.i2pc.es/index.php?title=2024Liu_Graphene&diff=4701&oldid=prevWikiSysop: Created page with "== Citation == Liu, Nan / Wang, Hong-Wei. Graphene in cryo-EM specimen optimization. 2024. Current Opinion in Structural Biology, Vol. 86, p. 102823 == Abstract == Specimen preparation is a critical but challenging step in highresolution cryogenic electron microscopy (cryo-EM) structural analysis of macromolecules. In the past decade, graphene has gained much recognition as the supporting substrate to optimize cryo-EM specimen preparation. It improves macromolecule em..."2024-08-16T06:16:39Z<p>Created page with "== Citation == Liu, Nan / Wang, Hong-Wei. Graphene in cryo-EM specimen optimization. 2024. Current Opinion in Structural Biology, Vol. 86, p. 102823 == Abstract == Specimen preparation is a critical but challenging step in highresolution cryogenic electron microscopy (cryo-EM) structural analysis of macromolecules. In the past decade, graphene has gained much recognition as the supporting substrate to optimize cryo-EM specimen preparation. It improves macromolecule em..."</p>
<p><b>New page</b></p><div>== Citation ==<br />
<br />
Liu, Nan / Wang, Hong-Wei. Graphene in cryo-EM specimen optimization. 2024. Current Opinion in Structural Biology, Vol. 86, p. 102823<br />
<br />
== Abstract ==<br />
<br />
Specimen preparation is a critical but challenging step in highresolution<br />
cryogenic electron microscopy (cryo-EM) structural<br />
analysis of macromolecules. In the past decade, graphene has<br />
gained much recognition as the supporting substrate to optimize<br />
cryo-EM specimen preparation. It improves macromolecule<br />
embedding in ice, reduces beam-induced motion, while<br />
imposing negligible background noise. Various types of<br />
graphene-coated cryo-EM grids were implemented to improve<br />
the robustness and efficiency of specimen preparation.<br />
Graphene functionalization by different means has been<br />
proved specifically useful in addressing challenges related to<br />
the air-water interface (AWI), such as preferential orientation<br />
and sample denaturation. Graphene sandwich specimen<br />
preparation sets a new direction to explore in cryo-EM analysis<br />
of biological specimens. In this review, we discuss the current<br />
challenges and future prospects of graphene application in<br />
cryo-EM analysis of macromolecules.<br />
<br />
== Keywords ==<br />
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== Links ==<br />
<br />
https://www.sciencedirect.com/science/article/pii/S0959440X24000502<br />
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== Related software ==<br />
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== Related methods ==<br />
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== Comments ==</div>WikiSysop