WikiSysop: Created page with "== Citation == Lytje, Kristian / Pedersen, Jan Skov. Validation of electron-microscopy maps using solution small-angle X-ray scattering. 2024. Acta Crystallographica Section D: Structural Biology, Vol. 80, No. 7, p. 493-505 == Abstract == The determination of the atomic resolution structure of biomacromolecules is essential for understanding details of their function. Traditionally, such a structure determination has been performed with crystallographic or nuclear res..."

2024-08-13T06:50:54Z

Created page with "== Citation == Lytje, Kristian / Pedersen, Jan Skov. Validation of electron-microscopy maps using solution small-angle X-ray scattering. 2024. Acta Crystallographica Section D: Structural Biology, Vol. 80, No. 7, p. 493-505 == Abstract == The determination of the atomic resolution structure of biomacromolecules is essential for understanding details of their function. Traditionally, such a structure determination has been performed with crystallographic or nuclear res..."

New page

== Citation ==

Lytje, Kristian / Pedersen, Jan Skov. Validation of electron-microscopy maps using solution small-angle X-ray scattering. 2024. Acta Crystallographica Section D: Structural Biology, Vol. 80, No. 7, p. 493-505

== Abstract ==

The determination of the atomic resolution structure of biomacromolecules
is essential for understanding details of their function. Traditionally, such a
structure determination has been performed with crystallographic or nuclear
resonance methods, but during the last decade, cryogenic transmission electron
microscopy (cryo-TEM) has become an equally important tool. As the blotting
and flash-freezing of the samples can induce conformational changes, external
validation tools are required to ensure that the vitrified samples are representative
of the solution. Although many validation tools have already been
developed, most of them rely on fully resolved atomic models, which prevents
early screening of the cryo-TEM maps. Here, a novel and automated method
for performing such a validation utilizing small-angle X-ray scattering
measurements, publicly available through the new software package AUSAXS,
is introduced and implemented. The method has been tested on both simulated
and experimental data, where it was shown to work remarkably well as a validation
tool. The method provides a dummy atomic model derived from the EM
map which best represents the solution structure.

== Keywords ==

== Links ==

https://journals.iucr.org/d/issues/2024/07/00/wan5003/

== Related software ==

== Related methods ==

== Comments ==

2024Lytje SAXS - Revision history