https://3demmethods.i2pc.es/index.php?action=history&feed=atom&title=2024Mastronarded_CTFPlotter 2026-05-24T20:20:13Z Revision history for this page on the wiki MediaWiki 1.44.2 https://3demmethods.i2pc.es/index.php?title=2024Mastronarded_CTFPlotter&diff=4586&oldid=prev 2024-07-31T06:29:25Z

<p>Created page with &quot;== Citation == Mastronarde, David N. Accurate, automatic determination of astigmatism and phase with Ctfplotter in IMOD. 2024. J. Structural Biology, Vol. 216, No. 1, p. 108057 == Abstract == Ctfplotter in the IMOD software package is a flexible program for determination of CTF parameters in tilt series images. It uses a novel approach to find astigmatism by measuring defocus in one-dimensional power spectra rotationally averaged over a series of restricted angular ra...&quot;</p> <p><b>New page</b></p><div>== Citation ==<br /> <br /> Mastronarde, David N. Accurate, automatic determination of astigmatism and phase with Ctfplotter in IMOD. 2024. J. Structural Biology, Vol. 216, No. 1, p. 108057<br /> <br /> == Abstract ==<br /> <br /> Ctfplotter in the IMOD software package is a flexible program for determination of CTF parameters in tilt series<br /> images. It uses a novel approach to find astigmatism by measuring defocus in one-dimensional power spectra<br /> rotationally averaged over a series of restricted angular ranges. Comparisons with Ctffind, Gctf, and Warp show<br /> that Ctfplotter’s estimated astigmatism is generally more reliable than that found by these programs that fit CTF<br /> parameters to two-dimensional power spectra, especially at higher tilt angles. In addition to that intrinsic<br /> advantage, Ctfplotter can reduce the variability in astigmatism estimates further by summing results over<br /> multiple tilt angles (typically 5), while still finding defocus for each individual image. Its fitting strategy also<br /> produces better phase estimates. The program now includes features for tuning the sampling of the power<br /> spectrum so that it is well-represented for analysis, and for determining an appropriate fitting range that can vary<br /> with tilt angle. It can thus be used automatically in a variety of situations, not just for fitting tilt series, and has<br /> been integrated into the SerialEM acquisition software for real-time determination of focus and astigmatism.<br /> <br /> == Keywords ==<br /> <br /> == Links ==<br /> <br /> https://www.sciencedirect.com/science/article/pii/S104784772300120X<br /> <br /> == Related software ==<br /> <br /> == Related methods ==<br /> <br /> == Comments ==</div>

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