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Created page with "== Citation == Mueller-Dieckmann, Christoph / Grinzato, Alessandro / Effantin, Grégory / Fenel, Daphna / Flot, David / Schoehn, Guy / Leonard, Gordon / Kandiah, Eaazhisai. From solution to structure: empowering inclusive cryo-EM with a pre-characterization pipeline for biological samples. 2024. J. Applied Crystallography, Vol. 57, No. 2 == Abstract == In addressing the challenges faced by laboratories and universities with limited (or no) cryo-electron microscopy (cr..."

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== Citation ==

Mueller-Dieckmann, Christoph / Grinzato, Alessandro / Effantin, Grégory / Fenel, Daphna / Flot, David / Schoehn, Guy / Leonard, Gordon / Kandiah, Eaazhisai. From solution to structure: empowering inclusive cryo-EM with a pre-characterization pipeline for biological samples. 2024. J. Applied Crystallography, Vol. 57, No. 2

== Abstract ==

In addressing the challenges faced by laboratories and universities with limited
(or no) cryo-electron microscopy (cryo-EM) infrastructure, the ESRF, in
collaboration with the Grenoble Institute for Structural Biology (IBS), has
implemented the cryo-EM Solution-to-Structure (SOS) pipeline. This inclusive
process, spanning grid preparation to high-resolution data collection, covers
single-particle analysis and cryo-electron tomography (cryo-ET). Accessible
through a rolling access route, proposals undergo scientific merit and technical
feasibility evaluations. Stringent feasibility criteria demand robust evidence of
sample homogeneity. Two distinct entry points are offered: users can either
submit purified protein samples for comprehensive processing or initiate the
pipeline with already vitrified cryo-EM grids. The SOS pipeline integrates
negative stain imaging (exclusive to protein samples) as a first quality step,
followed by cryo-EM grid preparation, grid screening and preliminary data
collection for single-particle analysis, or only the first two steps for cryo-ET. In
both cases, if the screening steps are successfully completed, high-resolution
data collection will be carried out using a Titan Krios microscope equipped with
a latest-generation direct electron counting detector coupled to an energy filter.
The SOS pipeline thus emerges as a comprehensive and efficient solution,
further democratizing access to cryo-EM research.

== Keywords ==

== Links ==

https://journals.iucr.org/j/issues/2024/02/00/ei5109/

== Related software ==

== Related methods ==

== Comments ==

2024Mueller Facility - Revision history