https://3demmethods.i2pc.es/index.php?action=history&feed=atom&title=2024Ren_Nucleosome 2026-05-24T20:41:28Z Revision history for this page on the wiki MediaWiki 1.44.2 https://3demmethods.i2pc.es/index.php?title=2024Ren_Nucleosome&diff=4827&oldid=prev 2024-11-12T08:01:23Z

<p></p> <table style="background-color: #fff; color: #202122;" data-mw="interface"> <col class="diff-marker" /> <col class="diff-content" /> <col class="diff-marker" /> <col class="diff-content" /> <tr class="diff-title" lang="en"> <td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">← Older revision</td> <td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 08:01, 12 November 2024</td> </tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l6">Line 6:</td> <td colspan="2" class="diff-lineno">Line 6:</td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Keywords ==</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Keywords ==</div></td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>Cryo-ET, single molecule structure, individual-particle cryo-electron tomography, IPET, nucleosome array, conformational <del style="font-weight: bold; text-decoration: none;">changesg</del></div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>Cryo-ET, single molecule structure, individual-particle cryo-electron tomography, IPET, nucleosome array, conformational <ins style="font-weight: bold; text-decoration: none;">changing</ins></div></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Paper links ==</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Paper links ==</div></td></tr> </table>

Garygangren https://3demmethods.i2pc.es/index.php?title=2024Ren_Nucleosome&diff=4825&oldid=prev 2024-11-12T07:56:36Z

<p>Created page with &quot;== Citation == Angle between DNA linker and nucleosome core particle regulates array compaction revealed by individual-particle cryo-electron tomography, Meng Zhang, César Díaz-Celis, Jianfang Liu, Jinhui Tao, Paul D. Ashby, Carlos Bustamante, Gang Ren, Nature Comm., (2024), 15, 4395. doi:10.1038/s41467-024-48305-1 == Abstract == The conformational dynamics of nucleosome arrays generate a diverse spectrum of microscopic states, posing challenges to their structural de...&quot;</p> <p><b>New page</b></p><div>== Citation ==<br /> Angle between DNA linker and nucleosome core particle regulates array compaction revealed by individual-particle cryo-electron tomography, Meng Zhang, César Díaz-Celis, Jianfang Liu, Jinhui Tao, Paul D. Ashby, Carlos Bustamante, Gang Ren, Nature Comm., (2024), 15, 4395. doi:10.1038/s41467-024-48305-1<br /> <br /> == Abstract ==<br /> The conformational dynamics of nucleosome arrays generate a diverse spectrum of microscopic states, posing challenges to their structural determination. Leveraging cryogenic electron tomography (cryo-ET), we determine the three-dimensional (3D) structures of individual mononucleosomes and arrays comprising di-, tri-, and tetranucleosomes. By slowing the rate of condensation through a reduction in ionic strength, we probe the intra-array structural transitions that precede inter-array interactions and liquid droplet formation. Under these conditions, the arrays exhibite irregular zig-zag conformations with loose packing. Increasing the ionic strength promoted intra-array compaction, yet we do not observe the previously reported regular 30-nanometer fibers. Interestingly, the presence of H1 do not induce array compaction; instead, one-third of the arrays display nucleosomes invaded by foreign DNA, suggesting an alternative role for H1 in chromatin network construction. We also find that the crucial parameter determining the structure adopted by chromatin arrays is the angle between the entry and exit of the DNA and the corresponding tangents to the nucleosomal disc. Our results provide insights into the initial stages of intra-array compaction, a critical precursor to condensation in the regulation of chromatin organization.<br /> <br /> == Keywords ==<br /> Cryo-ET, single molecule structure, individual-particle cryo-electron tomography, IPET, nucleosome array, conformational changesg<br /> <br /> == Paper links ==<br /> https://www.nature.com/articles/s41467-024-48305-1<br /> <br /> == Related video ==<br /> https://www.youtube.com/watch?v=z3LLknT14n4<br /> https://www.youtube.com/watch?v=e8USE0MecdU<br /> https://www.youtube.com/watch?v=RIFGnKzK0rk&amp;feature=youtu.be<br /> <br /> == Related methods ==<br /> https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0030249<br /> https://www.nature.com/articles/s41598-018-34652-9<br /> https://www.nature.com/articles/s41598-020-66793-1<br /> <br /> == Comments ==</div>

Garygangren