WikiSysop: Created page with "== Citation == R. Sanchez-Garcia, G. Gaullier, J. M. Cuadra-Troncoso, and J. Vargas, “Cryo-EM map anisotropy can be attenuated by map post-processing and a new method for its estimation,” Intl. J. Molecular Sciences, vol. 25, no. 7, p. 3959, 2024. == Abstract == One of the most important challenges in cryogenic electron microscopy (cryo-EM) is the substantial number of samples that exhibit preferred orientations, which leads to an uneven coverage of the projection..."

2024-10-18T06:40:17Z

Created page with "== Citation == R. Sanchez-Garcia, G. Gaullier, J. M. Cuadra-Troncoso, and J. Vargas, “Cryo-EM map anisotropy can be attenuated by map post-processing and a new method for its estimation,” Intl. J. Molecular Sciences, vol. 25, no. 7, p. 3959, 2024. == Abstract == One of the most important challenges in cryogenic electron microscopy (cryo-EM) is the substantial number of samples that exhibit preferred orientations, which leads to an uneven coverage of the projection..."

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== Citation ==

R. Sanchez-Garcia, G. Gaullier, J. M. Cuadra-Troncoso, and J. Vargas, “Cryo-EM map anisotropy can be attenuated by map post-processing and a new method for its estimation,” Intl. J. Molecular Sciences, vol. 25, no. 7, p. 3959, 2024.

== Abstract ==

One of the most important challenges in cryogenic electron microscopy (cryo-EM) is
the substantial number of samples that exhibit preferred orientations, which leads to an uneven
coverage of the projection sphere. As a result, the overall quality of the reconstructed maps can be
severely affected, as manifested by the presence of anisotropy in the map resolution. Several methods
have been proposed to measure the directional resolution of maps in tandem with experimental
protocols to address the problem of preferential orientations in cryo-EM. Following these works, in
this manuscript we identified one potential limitation that may affect most of the existing methods
and we proposed an alternative approach to evaluate the presence of preferential orientations in
cryo-EM reconstructions. In addition, we also showed that some of the most recently proposed
cryo-EM map post-processing algorithms can attenuate map anisotropy, thus offering alternative
visualization opportunities for cases affected by moderate levels of preferential orientations.

== Keywords ==

== Links ==

https://www.mdpi.com/1422-0067/25/7/3959

== Related software ==

== Related methods ==

== Comments ==

2024Sanchez Anisotropy - Revision history