https://3demmethods.i2pc.es/index.php?action=history&feed=atom&title=2024Schiotz_Review2024Schiotz Review - Revision history2026-06-13T12:18:06ZRevision history for this page on the wikiMediaWiki 1.44.2https://3demmethods.i2pc.es/index.php?title=2024Schiotz_Review&diff=4859&oldid=prevWikiSysop: Created page with "== Citation == O. H. Schiøtz, S. Klumpe, J. M. Plitzko, and C. J. Kaiser, “Cryo-electron tomography: en route to the molecular anatomy of organisms and tissues,” Biochemical Society Transactions, p. BST20240173, 2024. == Abstract == Cryo-electron tomography (cryo-ET) has become a key technique for obtaining structures of macromolecular complexes in their native environment, assessing their local organization and describing the molecular sociology of the cell. Whi..."2024-12-12T08:04:50Z<p>Created page with "== Citation == O. H. Schiøtz, S. Klumpe, J. M. Plitzko, and C. J. Kaiser, “Cryo-electron tomography: en route to the molecular anatomy of organisms and tissues,” Biochemical Society Transactions, p. BST20240173, 2024. == Abstract == Cryo-electron tomography (cryo-ET) has become a key technique for obtaining structures of macromolecular complexes in their native environment, assessing their local organization and describing the molecular sociology of the cell. Whi..."</p>
<p><b>New page</b></p><div>== Citation ==<br />
<br />
O. H. Schiøtz, S. Klumpe, J. M. Plitzko, and C. J. Kaiser, “Cryo-electron tomography: en route to the molecular anatomy of organisms and tissues,” Biochemical Society Transactions, p. BST20240173, 2024.<br />
<br />
== Abstract ==<br />
<br />
Cryo-electron tomography (cryo-ET) has become a key technique for obtaining structures<br />
of macromolecular complexes in their native environment, assessing their local organization<br />
and describing the molecular sociology of the cell. While microorganisms and adherent<br />
mammalian cells are common targets for tomography studies, appropriate sample preparation<br />
and data acquisition strategies for larger cellular assemblies such as tissues, organoids<br />
or small model organisms have only recently become sufficiently practical to allow<br />
for in-depth structural characterization of such samples in situ. These advances include tailored<br />
lift-out approaches using focused ion beam (FIB) milling, and improved data acquisition<br />
schemes. Consequently, cryo-ET of FIB lamellae from large volume samples can<br />
complement ultrastructural analysis with another level of information: molecular anatomy.<br />
This review highlights the recent developments towards molecular anatomy studies using<br />
cryo-ET, and briefly outlines what can be expected in the near future.<br />
<br />
== Keywords ==<br />
<br />
== Links ==<br />
<br />
https://portlandpress.com/biochemsoctrans/article/doi/10.1042/BST20240173/235350/Cryo-electron-tomography-en-route-to-the-molecular<br />
<br />
== Related software ==<br />
<br />
== Related methods ==<br />
<br />
== Comments ==</div>WikiSysop