https://3demmethods.i2pc.es/index.php?action=history&feed=atom&title=2024Schwab_DynaMight 2026-06-13T13:45:53Z Revision history for this page on the wiki MediaWiki 1.44.2 https://3demmethods.i2pc.es/index.php?title=2024Schwab_DynaMight&diff=4721&oldid=prev 2024-08-21T06:53:02Z

<p>Created page with &quot;== Citation == Schwab, Johannes / Kimanius, Dari / Burt, Alister / Dendooven, Tom / Scheres, Sjors H. W. DynaMight: estimating molecular motions with improved reconstruction from cryo-EM images. 2024. Nature Methods, p. 1-8 == Abstract == How to deal with continuously flexing molecules is one of the biggest outstanding challenges in single-particle analysis of proteins from cryogenic-electron microscopy (cryo-EM) images. Here, we present DynaMight, a software tool tha...&quot;</p> <p><b>New page</b></p><div>== Citation ==<br /> <br /> Schwab, Johannes / Kimanius, Dari / Burt, Alister / Dendooven, Tom / Scheres, Sjors H. W. DynaMight: estimating molecular motions with improved reconstruction from cryo-EM images. 2024. Nature Methods, p. 1-8<br /> <br /> == Abstract ==<br /> <br /> How to deal with continuously flexing molecules is one of the biggest<br /> outstanding challenges in single-particle analysis of proteins from<br /> cryogenic-electron microscopy (cryo-EM) images. Here, we present<br /> DynaMight, a software tool that estimates a continuous space of<br /> conformations in a cryo-EM dataset by learning three-dimensional<br /> deformations of a Gaussian pseudo-atomic model of a consensus structure<br /> for every particle image. Inversion of the learned deformations is then<br /> used to obtain an improved reconstruction of the consensus structure. We<br /> illustrate the performance of DynaMight for several experimental cryo-EM<br /> datasets. We also show how error estimates on the deformations may be<br /> obtained by independently training two variational autoencoders on half<br /> sets of the cryo-EM data, and how regularization of the three-dimensional<br /> deformations through the use of atomic models may lead to important<br /> artifacts due to model bias. DynaMight is distributed as free, open-source<br /> software, as part of RELION-5.<br /> <br /> == Keywords ==<br /> <br /> == Links ==<br /> <br /> https://www.nature.com/articles/s41592-024-02377-5<br /> <br /> == Related software ==<br /> <br /> == Related methods ==<br /> <br /> == Comments ==</div>

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