WikiSysop: Created page with "== Citation == Wan, William / Khavnekar, Sagar / Wagner, Jonathan. STOPGAP: an open-source package for template matching, subtomogram alignment and classification. 2024. Acta Crystallographica Section D: Structural Biology, Vol. 80, No. 5 == Abstract == Cryo-electron tomography (cryo-ET) enables molecular-resolution 3D imaging of complex biological specimens such as viral particles, cellular sections and, in some cases, whole cells. This enables the structural charact..."

2024-08-09T06:24:50Z

Created page with "== Citation == Wan, William / Khavnekar, Sagar / Wagner, Jonathan. STOPGAP: an open-source package for template matching, subtomogram alignment and classification. 2024. Acta Crystallographica Section D: Structural Biology, Vol. 80, No. 5 == Abstract == Cryo-electron tomography (cryo-ET) enables molecular-resolution 3D imaging of complex biological specimens such as viral particles, cellular sections and, in some cases, whole cells. This enables the structural charact..."

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== Citation ==

Wan, William / Khavnekar, Sagar / Wagner, Jonathan. STOPGAP: an open-source package for template matching, subtomogram alignment and classification. 2024. Acta Crystallographica Section D: Structural Biology, Vol. 80, No. 5

== Abstract ==

Cryo-electron tomography (cryo-ET) enables molecular-resolution 3D imaging
of complex biological specimens such as viral particles, cellular sections and, in
some cases, whole cells. This enables the structural characterization of molecules
in their near-native environments, without the need for purification or separation,
thereby preserving biological information such as conformational states
and spatial relationships between different molecular species. Subtomogram
averaging is an image-processing workflow that allows users to leverage cryo-ET
data to identify and localize target molecules, determine high-resolution structures
of repeating molecular species and classify different conformational states.
Here, STOPGAP, an open-source package for subtomogram averaging that is
designed to provide users with fine control over each of these steps, is described.
In providing detailed descriptions of the image-processing algorithms that
STOPGAP uses, this manuscript is also intended to serve as a technical resource
to users as well as for further community-driven software development.

== Keywords ==

== Links ==

https://journals.iucr.org/d/issues/2024/05/00/vo5015/

== Related software ==

== Related methods ==

== Comments ==

2024Wan StopGap - Revision history