WikiSysop: Created page with "== Citation == Wang, X., Jin, Q., Zou, L., Lin, X. and Lu, Y. 2024. Orientation Determination of Cryo-EM Projection Images Using Reliable Common Lines and Spherical Embeddings. IEEE/ACM Transactions on Computational Biology and Bioinformatics. (2024). == Abstract == Three-dimensional (3D) reconstruction in singleparticle cryo-electronmicroscopy (cryo-EM) is a critical technique for recovering and studying the fine 3D structure of proteins and other biological macromol..."

2025-11-14T17:03:09Z

Created page with "== Citation == Wang, X., Jin, Q., Zou, L., Lin, X. and Lu, Y. 2024. Orientation Determination of Cryo-EM Projection Images Using Reliable Common Lines and Spherical Embeddings. IEEE/ACM Transactions on Computational Biology and Bioinformatics. (2024). == Abstract == Three-dimensional (3D) reconstruction in singleparticle cryo-electronmicroscopy (cryo-EM) is a critical technique for recovering and studying the fine 3D structure of proteins and other biological macromol..."

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== Citation ==

Wang, X., Jin, Q., Zou, L., Lin, X. and Lu, Y. 2024. Orientation Determination of Cryo-EM Projection Images Using Reliable Common Lines and Spherical Embeddings. IEEE/ACM Transactions on Computational Biology and Bioinformatics. (2024).

== Abstract ==

Three-dimensional (3D) reconstruction in singleparticle
cryo-electronmicroscopy (cryo-EM) is a critical technique
for recovering and studying the fine 3D structure of proteins and
other biological macromolecules, where the primary issue is to
determine the orientations of projection images with high levels
of noise. This paper proposes a method to determine the orientations
of cryo-EM projection images using reliable common lines
and spherical embeddings. First, the reliability of common lines
between projection images is evaluated using a weighted voting
algorithm based on an iterative improvement technique and binarized
weighting. Then, the reliable common lines are used to
calculate the normal vectors and localX-axis vectors of projection
images after two spherical embeddings. Finally, the orientations of
projection images are determined by aligning the results of the two
spherical embeddings using an orthogonal constraint. Experimental
results on both synthetic and real cryo-EM projection image
datasets demonstrate that the proposed method can achieve higher
accuracy in estimating the orientations of projection images and
higher resolution in reconstructing preliminary 3D structures than
some common line-based methods, indicating that the proposed
method is effective in single-particle cryo-EM 3D reconstruction.

== Keywords ==

== Links ==

https://ieeexplore.ieee.org/abstract/document/10711260

== Related software ==

== Related methods ==

== Comments ==

2024Wang CommonLines - Revision history