https://3demmethods.i2pc.es/index.php?action=history&feed=atom&title=2024Zhang_CTFMeasure 2026-06-13T12:38:42Z Revision history for this page on the wiki MediaWiki 1.44.2 https://3demmethods.i2pc.es/index.php?title=2024Zhang_CTFMeasure&diff=4717&oldid=prev 2024-08-21T06:14:16Z

<p>Created page with &quot;== Citation == Zhang, Ranhao / Shen, Yuan / Li, Xueming. Tilt-series-based joint CTF estimation for cryo-electron tomography. 2024. Structure, Vol. 32, p. 1239-1247 == Abstract == Contrast transfer function (CTF) estimation is a necessary step in the cryo-electron tomography (cryoET) workflow and essential for high-resolution in situ structural determination. However, the low signal-to-noise ratio and continuous defocus variation in micrographs of cryoET tilt series m...&quot;</p> <p><b>New page</b></p><div>== Citation ==<br /> <br /> Zhang, Ranhao / Shen, Yuan / Li, Xueming. Tilt-series-based joint CTF estimation for cryo-electron tomography. 2024. Structure, Vol. 32, p. 1239-1247<br /> <br /> == Abstract ==<br /> <br /> Contrast transfer function (CTF) estimation is a necessary step in the cryo-electron tomography (cryoET)<br /> workflow and essential for high-resolution in situ structural determination. However, the low signal-to-noise<br /> ratio and continuous defocus variation in micrographs of cryoET tilt series make accurate CTF estimation<br /> challenging. Here, we report a tilt-series-based joint CTF estimation method implemented in the new software<br /> CTFMeasure. The joint estimation method combines all Thon-ring signals in a tilt series to improve<br /> the estimation accuracy. By using an objective function involving the CTF parameters and geometric<br /> parameters of a cryoET tilt series, CTFMeasure can estimate the CTF parameters of each micrograph and<br /> the absolute tilt angle offset of the lamellar sample relative to the sample stage plane, which is usually the<br /> glancing angle used during focused ion beam (FIB) milling. Tests on both synthetic and experimental data,<br /> as well as subtomogram averaging, demonstrated the accurate CTF estimation of cryoET tilt series by<br /> CTFMeasure.<br /> <br /> == Keywords ==<br /> <br /> == Links ==<br /> <br /> https://www.cell.com/structure/fulltext/S0969-2126(24)00183-7<br /> <br /> == Related software ==<br /> <br /> == Related methods ==<br /> <br /> == Comments ==</div>

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