https://3demmethods.i2pc.es/index.php?action=history&feed=atom&title=2024Zhu_SIRM 2026-05-01T15:10:53Z Revision history for this page on the wiki MediaWiki 1.44.2 https://3demmethods.i2pc.es/index.php?title=2024Zhu_SIRM&diff=4705&oldid=prev 2024-08-20T06:07:37Z

<p>Created page with &quot;== Citation == Zhu, Dongjie / Cao, Weili / Li, Junxi / Wu, Chunling / Cao, Duanfang / Zhang, Xinzheng. Correction of preferred orientation--induced distortion in cryo-electron microscopy maps. 2024. Science Advances, Vol. 10, No. 30, p. eadn0092 == Abstract == Reconstruction maps of cryo–electron microscopy (cryo-EM) exhibit distortion when the cryo-EM dataset is incomplete, usually caused by unevenly distributed orientations. Prior efforts had been attempted to ad...&quot;</p> <p><b>New page</b></p><div>== Citation ==<br /> <br /> Zhu, Dongjie / Cao, Weili / Li, Junxi / Wu, Chunling / Cao, Duanfang / Zhang, Xinzheng. Correction of preferred orientation--induced distortion in cryo-electron microscopy maps. <br /> 2024. Science Advances, Vol. 10, No. 30, p. eadn0092<br /> <br /> == Abstract ==<br /> <br /> Reconstruction maps of cryo–electron microscopy (cryo-EM)<br /> exhibit distortion when the cryo-EM<br /> dataset is incomplete,<br /> usually caused by unevenly distributed orientations. Prior efforts had been attempted to address this<br /> preferred orientation problem using tilt-collection<br /> strategy and modifications to grids or to air-water<br /> interfaces.<br /> However, these approaches often require time-consuming<br /> experiments, and the effect was always protein dependent.<br /> Here, we developed a procedure containing removing misaligned particles and an iterative reconstruction<br /> method based on signal-to-<br /> noise<br /> ratio of Fourier component to correct this distortion by recovering missing data<br /> using a purely computational algorithm. This procedure called signal-to-<br /> noise<br /> ratio iterative reconstruction method<br /> (SIRM) was applied on incomplete datasets of various proteins to fix distortion in cryo-EM<br /> maps and to a more<br /> isotropic resolution. In addition, SIRM provides a better reference map for further reconstruction refinements,<br /> resulting in an improved alignment, which ultimately improves map quality and benefits model building.<br /> <br /> == Keywords ==<br /> <br /> == Links ==<br /> <br /> https://www.science.org/doi/full/10.1126/sciadv.adn0092<br /> <br /> == Related software ==<br /> <br /> == Related methods ==<br /> <br /> == Comments ==</div>

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