https://3demmethods.i2pc.es/index.php?action=history&feed=atom&title=2024vanBlerkom_GoldX 2026-06-13T12:18:04Z Revision history for this page on the wiki MediaWiki 1.44.2 https://3demmethods.i2pc.es/index.php?title=2024vanBlerkom_GoldX&diff=4617&oldid=prev 2024-08-07T05:50:02Z

<p>Created page with &quot;== Citation == Van Blerkom, Peter / Bezault, Armel / Sauvanet, Cécile / Hanein, Dorit / Volkmann, Niels. The GoldX Fiducial Eraser. 2024. Intl. J. Molecular Sciences, Vol. 25, No. 13 == Abstract == Gold nanoparticles with sizes in the range of 5–15 nm are a standard method of providing fiducial markers to assist with alignment during reconstruction in cryogenic electron tomography. However, due to their high electron density and resulting contrast when compared to...&quot;</p> <p><b>New page</b></p><div>== Citation ==<br /> <br /> Van Blerkom, Peter / Bezault, Armel / Sauvanet, Cécile / Hanein, Dorit / Volkmann, Niels. The GoldX Fiducial Eraser. 2024. Intl. J. Molecular Sciences, Vol. 25, No. 13<br /> <br /> == Abstract ==<br /> <br /> Gold nanoparticles with sizes in the range of 5–15 nm are a standard method of providing fiducial markers to assist with alignment during reconstruction in cryogenic electron tomography. However, due to their high electron density and resulting contrast when compared to standard cellular or biological samples, they introduce artifacts such as streaking in the reconstructed tomograms. Here, we demonstrate a tool that automatically detects these nanoparticles and suppresses them by replacing them with a local background as a post-processing step, providing a cleaner tomogram without removing any sample relevant information or introducing new artifacts or edge effects from uniform density replacements.<br /> <br /> == Keywords ==<br /> <br /> == Links ==<br /> <br /> https://www.mdpi.com/1422-0067/25/13/7442<br /> <br /> == Related software ==<br /> <br /> == Related methods ==<br /> <br /> == Comments ==</div>

WikiSysop