WikiSysop: Created page with "== Citation == Chen, W., Zheng, J., Zhou, J., Cheng, L. and Liu, H. 2025. A data-processing strategy of asymmetric reconstruction for tailed phages by Cryo-electron Microscopy. J. Structural Biology. (2025), 108262. == Abstract == The structure of the tailed phage is composed of an icosahedral (or elongated icosahedral) head and a spiral symmetrical tail, which are connected by a portal located at a unique vertex of the icosahedron. A series of image-processing method..."

2025-12-29T08:50:55Z

Created page with "== Citation == Chen, W., Zheng, J., Zhou, J., Cheng, L. and Liu, H. 2025. A data-processing strategy of asymmetric reconstruction for tailed phages by Cryo-electron Microscopy. J. Structural Biology. (2025), 108262. == Abstract == The structure of the tailed phage is composed of an icosahedral (or elongated icosahedral) head and a spiral symmetrical tail, which are connected by a portal located at a unique vertex of the icosahedron. A series of image-processing method..."

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== Citation ==

Chen, W., Zheng, J., Zhou, J., Cheng, L. and Liu, H. 2025. A data-processing strategy of asymmetric reconstruction for tailed phages by Cryo-electron Microscopy. J. Structural Biology. (2025), 108262.

== Abstract ==

The structure of the tailed phage is composed of an icosahedral (or elongated icosahedral) head and a spiral
symmetrical tail, which are connected by a portal located at a unique vertex of the icosahedron. A series of
image-processing methods and tools have been developed to address the asymmetric structures of phages.
However, the structural determination in small proteins within the head and flexible proteins of tailed phages
remains a significant impediment, further hindering our deep understanding of the structural biology field. In
this study, we developed a data-processing strategy for tailed phage and demonstrated its efficacy with three
cryo-EM datasets, including podophage T7, siphophage T1, and myophage Mu. The proposed strategy combines
conventional icosahedral reconstruction with local refinement and reconstruction and consists of four key
modules: icosahedral reconstruction, selection of the unique vertex of the icosahedron, local asymmetric
reconstruction and refinement, and local defocus refinement. The strategy has been successfully applied to
determine the asymmetric structure of a range of tailed phages, with a particular focus on resolving the small
proteins (core proteins and scaffolding proteins) within the head and flexible proteins on the tail. In addition, the
local defocus refinement of our strategy approaches the approximate resolution limit of the icosahedral capsid.
The proposed strategy is a viable scheme for determining the asymmetric structures of tailed phages, especially
in podophages.

== Keywords ==

== Links ==

https://www.sciencedirect.com/science/article/pii/S1047847725000978

== Related software ==

== Related methods ==

== Comments ==

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