WikiSysop: Created page with "== Citation == Elad, S.T., Ben-Asher, N. and Engel, L. 2025. Cool and collected: Advances in sample preparation for cryo-electron microscopy. Current Opinion in Structural Biology. 94, (2025), 103132. == Abstract == Cryo-electron microscopy (cryo-EM) has emerged as a transformative tool in structural biology, enabling highresolution visualization of macromolecules in their native states. Cryo-focused ion beam milling (cryo-FIB) and other advances in sample preparation..."

2025-11-02T22:43:20Z

Created page with "== Citation == Elad, S.T., Ben-Asher, N. and Engel, L. 2025. Cool and collected: Advances in sample preparation for cryo-electron microscopy. Current Opinion in Structural Biology. 94, (2025), 103132. == Abstract == Cryo-electron microscopy (cryo-EM) has emerged as a transformative tool in structural biology, enabling highresolution visualization of macromolecules in their native states. Cryo-focused ion beam milling (cryo-FIB) and other advances in sample preparation..."

New page

== Citation ==

Elad, S.T., Ben-Asher, N. and Engel, L. 2025. Cool and collected: Advances in sample preparation for cryo-electron microscopy. Current Opinion in Structural Biology. 94, (2025), 103132.

== Abstract ==

Cryo-electron microscopy (cryo-EM) has emerged as a
transformative tool in structural biology, enabling highresolution
visualization of macromolecules in their native
states. Cryo-focused ion beam milling (cryo-FIB) and other
advances in sample preparation have expanded the range of
biological samples that can be studied with cryo-EM to include
cells and tissues. While the dream of high-resolution structural
analysis of proteins within their native, cellular context is now
being realized, sample preparation, especially from tissues, is
still labor-intensive and technically challenging. Here we
review the latest innovations in cryo-EM sample preparation,
including support fabrication and functionalization, cell
micropatterning, and techniques for thinning frozen biological
samples. Beyond streamlining and improving the repeatability
of sample preparation, these advances are expanding the
impact of cryo-EM by enabling unprecedented visualization of
structures within cells and tissues in healthy and diseased
states, as well as structural analysis of biological processes at
well-controlled time points.

== Keywords ==

== Links ==

https://www.sciencedirect.com/science/article/pii/S0959440X25001502

== Related software ==

== Related methods ==

== Comments ==

2025Elad Review - Revision history