WikiSysop: Created page with "== Citation == Grant, A.J. and Schmidt-Krey, I. 2025. Nanodisc-reconstitution for single particle cryo-EM structure determination of membrane proteins. Current Opinion in Structural Biology. 93, (2025), 103072. == Abstract == Reconstitution of membrane proteins in nanodiscs has proven to be a highly effective approach to study membrane protein structures in a lipid bilayer, resulting in many recent single particle cryo-EM structures. While most of these studies employ..."

2025-08-28T09:57:31Z

Created page with "== Citation == Grant, A.J. and Schmidt-Krey, I. 2025. Nanodisc-reconstitution for single particle cryo-EM structure determination of membrane proteins. Current Opinion in Structural Biology. 93, (2025), 103072. == Abstract == Reconstitution of membrane proteins in nanodiscs has proven to be a highly effective approach to study membrane protein structures in a lipid bilayer, resulting in many recent single particle cryo-EM structures. While most of these studies employ..."

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== Citation ==

Grant, A.J. and Schmidt-Krey, I. 2025. Nanodisc-reconstitution for single particle cryo-EM structure determination of membrane proteins. Current Opinion in Structural Biology. 93, (2025), 103072.

== Abstract ==

Reconstitution of membrane proteins in nanodiscs has proven
to be a highly effective approach to study membrane protein
structures in a lipid bilayer, resulting in many recent single
particle cryo-EM structures. While most of these studies
employed membrane scaffold protein (MSP) nanodiscs, additional
types of nanodiscs were developed based on MSPs and
provide alternative approaches. Nanodiscs have been particularly
effective in solving structures of different protein conformations
and of bound lipids, demonstrating key roles of
specific lipids in structural integrity and protein function. At the
same time, discrepancies of lipid behavior in nanodiscs
compared to native membranes and liposomes necessitate
careful scrutiny of reconstitution parameters and further evaluation.
This brief review covers an overview of types of
nanodiscs currently in use for cryo-EM structural studies, their
advantages and limitations, as well as examples of the
dramatically increased understanding they can reveal.

== Keywords ==

== Links ==

https://www.sciencedirect.com/science/article/pii/S0959440X25000909

== Related software ==

== Related methods ==

== Comments ==

2025Grant Nanodisc - Revision history