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Created page with "== Citation == Pintilie, G., Shao, C., Wang, Z., Hudson, B.P., Flatt, J.W., Schmid, M.F., Morris, K., Burley, S.K. and Chiu, W. 2025. Q-score as a reliability measure for protein, nucleic acid, and small molecule atomic coordinate models derived from 3DEM density maps. Structure. D81, (2025), 410–422. == Abstract == Atomic coordinate models are important for the interpretation of 3D maps produced with cryoEM and cryoET (3D electron microscopy; 3DEM). In addition to..."

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== Citation ==

Pintilie, G., Shao, C., Wang, Z., Hudson, B.P., Flatt, J.W., Schmid, M.F., Morris, K., Burley, S.K. and Chiu, W. 2025. Q-score as a reliability measure for protein, nucleic acid, and small molecule atomic coordinate models derived from 3DEM density maps. Structure. D81, (2025), 410–422.

== Abstract ==

Atomic coordinate models are important for the interpretation of 3D maps
produced with cryoEM and cryoET (3D electron microscopy; 3DEM). In
addition to visual inspection of such maps and models, quantitative metrics can
inform about the reliability of the atomic coordinates, in particular how well the
model is supported by the experimentally determined 3DEM map. A recently
introduced metric, Q-score, was shown to correlate well with the reported
resolution of the map for well fitted models. Here, we present new statistical
analyses of Q-score based on its application to 10 000 maps and models
archived in the EMDB (Electron Microscopy Data Bank) and PDB (Protein
Data Bank). Further, we introduce two new metrics based on Q-score to
represent each map and model relative to all entries in the EMDB and those
with similar resolution. We explore through illustrative examples of proteins,
nucleic acids and small molecules how Q-scores can indicate whether the atomic
coordinates are well fitted to 3DEM maps and also whether some parts of a map
may be poorly resolved due to factors such as molecular flexibility, radiation
damage and/or conformational heterogeneity. These examples and statistical
analyses provide a basis for how Q-scores can be interpreted effectively in order
to evaluate 3DEM maps and atomic coordinate models prior to publication and
archiving.

== Keywords ==

== Links ==

https://journals.iucr.org/d/issues/2025/08/00/ic5125/index.html

== Related software ==

== Related methods ==

== Comments ==

2025Pintilie QScore - Revision history