https://3demmethods.i2pc.es/index.php?action=history&feed=atom&title=2025Sun_PlasmaMembranes2025Sun PlasmaMembranes - Revision history2026-05-24T21:14:21ZRevision history for this page on the wikiMediaWiki 1.44.2https://3demmethods.i2pc.es/index.php?title=2025Sun_PlasmaMembranes&diff=4931&oldid=prevWikiSysop: Created page with "== Citation == W. W. Sun et al., “Cryo-electron tomography pipeline for plasma membranes,” Nature Communications, vol. 16, no. 1, p. 855, 2025. == Abstract == Cryo-electron tomography (cryoET) provides sub-nanometer protein structure within the dense cellular environment. Existing sample preparation methods are insufficient at accessing the plasma membrane and its associated proteins. Here, we present a correlative cryo-electron tomography pipeline optimally suite..."2025-02-03T07:37:47Z<p>Created page with "== Citation == W. W. Sun et al., “Cryo-electron tomography pipeline for plasma membranes,” Nature Communications, vol. 16, no. 1, p. 855, 2025. == Abstract == Cryo-electron tomography (cryoET) provides sub-nanometer protein structure within the dense cellular environment. Existing sample preparation methods are insufficient at accessing the plasma membrane and its associated proteins. Here, we present a correlative cryo-electron tomography pipeline optimally suite..."</p>
<p><b>New page</b></p><div>== Citation ==<br />
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W. W. Sun et al., “Cryo-electron tomography pipeline for plasma membranes,” Nature Communications, vol. 16, no. 1, p. 855, 2025.<br />
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== Abstract ==<br />
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Cryo-electron tomography (cryoET) provides sub-nanometer protein structure<br />
within the dense cellular environment. Existing sample preparation<br />
methods are insufficient at accessing the plasma membrane and its associated<br />
proteins. Here, we present a correlative cryo-electron tomography pipeline<br />
optimally suited to image large ultra-thin areas of isolated basal and apical<br />
plasma membranes. The pipeline allows for angstrom-scale structure determination<br />
with subtomogram averaging and employs a genetically encodable<br />
rapid chemically-induced electron microscopy visible tag for marking specific<br />
proteins within the complex cellular environment. The pipeline provides efficient,<br />
distributable, low-cost sample preparation and enables targeted structural<br />
studies of identified proteins at the plasma membrane of<br />
mammalian cells.<br />
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== Keywords ==<br />
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== Links ==<br />
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https://www.nature.com/articles/s41467-025-56045-z<br />
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== Related software ==<br />
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== Related methods ==<br />
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== Comments ==</div>WikiSysop