WikiSysop: Created page with "== Citation == Zhang, K., Cossio, P., Rangan, A.V., Lucas, B.A. and Grigorieff, N. 2025. A new statistical metric for robust target detection in cryo-EM using 2D template matching. IUCrJ. 12, 2 (2025). == Abstract == 2D template matching (2DTM) can be used to detect molecules and their assemblies in cellular cryo-EM images with high positional and orientational accuracy. While 2DTM successfully detects spherical targets such as large ribosomal subunits, challenges rem..."

2025-08-20T09:54:15Z

Created page with "== Citation == Zhang, K., Cossio, P., Rangan, A.V., Lucas, B.A. and Grigorieff, N. 2025. A new statistical metric for robust target detection in cryo-EM using 2D template matching. IUCrJ. 12, 2 (2025). == Abstract == 2D template matching (2DTM) can be used to detect molecules and their assemblies in cellular cryo-EM images with high positional and orientational accuracy. While 2DTM successfully detects spherical targets such as large ribosomal subunits, challenges rem..."

New page

== Citation ==

Zhang, K., Cossio, P., Rangan, A.V., Lucas, B.A. and Grigorieff, N. 2025. A new statistical metric for robust target detection in cryo-EM using 2D template matching. IUCrJ. 12, 2 (2025).

== Abstract ==

2D template matching (2DTM) can be used to detect molecules and their
assemblies in cellular cryo-EM images with high positional and orientational
accuracy. While 2DTM successfully detects spherical targets such as large
ribosomal subunits, challenges remain in detecting smaller and more aspherical
targets in various environments. In this work, a novel 2DTM metric, referred to
as the 2DTM p-value, is developed to extend the 2DTM framework to more
complex applications. The 2DTM p-value combines information from two
previously used 2DTM metrics, namely the 2DTM signal-to-noise ratio (SNR)
and z-score, which are derived from the cross-correlation coefficient between
the target and the template. The 2DTM p-value demonstrates robust detection
accuracies under various imaging and sample conditions and outperforms the
2DTM SNR and z-score alone. Specifically, the 2DTM p-value improves the
detection of aspherical targets such as a modified artificial tubulin patch particle
(500 kDa) and a much smaller clathrin monomer (193 kDa) in simulated data. It
also accurately recovers mature 60S ribosomes in yeast lamellae samples, even
under conditions of increased Gaussian noise. The new metric will enable the
detection of a wider variety of targets in both purified and cellular samples
through 2DTM.

== Keywords ==

== Links ==

https://journals.iucr.org/m/issues/2025/02/00/eh5020/index.html

== Related software ==

== Related methods ==

== Comments ==

2025Zhang 2DTMpValue - Revision history