WikiSysop: Created page with "== Citation == Barchet, C., von Loeffelholz, O., Bahena-Ceron, R., Klaholz, B.P. and Urzhumtsev, A.G. 2026. Explicit correction of severely non-uniform distributions of cryo-EM views. Biological Crystallography. 82, 2 (2026). == Abstract == The quality of three-dimensional macromolecular image reconstruction by cryo electron microscopy (cryo-EM) strongly depends on the number and the quality of the respective two-dimensional projections and on their angular distributi..."

2026-02-17T04:30:10Z

Created page with "== Citation == Barchet, C., von Loeffelholz, O., Bahena-Ceron, R., Klaholz, B.P. and Urzhumtsev, A.G. 2026. Explicit correction of severely non-uniform distributions of cryo-EM views. Biological Crystallography. 82, 2 (2026). == Abstract == The quality of three-dimensional macromolecular image reconstruction by cryo electron microscopy (cryo-EM) strongly depends on the number and the quality of the respective two-dimensional projections and on their angular distributi..."

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== Citation ==

Barchet, C., von Loeffelholz, O., Bahena-Ceron, R., Klaholz, B.P. and Urzhumtsev, A.G. 2026. Explicit correction of severely non-uniform distributions of cryo-EM views. Biological Crystallography. 82, 2 (2026).

== Abstract ==

The quality of three-dimensional macromolecular image reconstruction by cryo
electron microscopy (cryo-EM) strongly depends on the number and the quality
of the respective two-dimensional projections and on their angular distribution
in space. Distributions with one or a few strongly preferred particle orientations
may result in maps that are deformed in certain directions. A simple removal of
overrepresented views may improve the quality of the reconstructed maps when
the level of noise in the two-dimensional (2D) projections is low and the data-set
size can afford this removal, but is counterproductive otherwise. Complementarily,
giving an increased weight to underrepresented views, or taking
multiple copies of them during the reconstruction, may improve the results,
naturally, depending on how non-uniform the view distribution is. This work
describes the results of three-dimensional (3D) reconstructions using an explicit
correction of the number of overrepresented and underrepresented projections
for non-uniformly distributed sets. Such correction can be considered as a
potential preprocessing, fast and simple, during 3D reconstruction in the imageprocessing
and cryo-EM structure-determination workflow.

== Keywords ==

== Links ==

https://journals.iucr.org/d/issues/2026/02/00/sor5004/index.html

== Related software ==

== Related methods ==

== Comments ==

2026Barchet NonUniform - Revision history