https://3demmethods.i2pc.es/index.php?action=history&feed=atom&title=2026Burton_PASR 2026-09-18T10:36:31Z Revision history for this page on the wiki MediaWiki 1.44.2 https://3demmethods.i2pc.es/index.php?title=2026Burton_PASR&diff=5281&oldid=prev 2026-09-18T05:08:06Z

<p>Created page with &quot;== Citation == Burton Smith, R. and Murata, K. 2026. Post-acquisition super resolution for cryo-electron microscopy. IUCrJ. 13, 5 (2026). == Abstract == Recently, reports have demonstrated achieving resolutions beyond the physical Nyquist limit using super resolution acquisition. Here, we demonstrate exceeding this limitation by pre-processing the raw micrograph movies from counting mode data that have already reached the physical Nyquist reconstruction resolution. To...&quot;</p> <p><b>New page</b></p><div>== Citation ==<br /> <br /> Burton Smith, R. and Murata, K. 2026. Post-acquisition super resolution for cryo-electron microscopy. IUCrJ. 13, 5 (2026).<br /> <br /> == Abstract ==<br /> <br /> Recently, reports have demonstrated achieving resolutions beyond the physical<br /> Nyquist limit using super resolution acquisition. Here, we demonstrate<br /> exceeding this limitation by pre-processing the raw micrograph movies from<br /> counting mode data that have already reached the physical Nyquist reconstruction<br /> resolution. To demonstrate functionality, micrograph movies of<br /> multiple datasets were pre-processed, showing that it is possible to exceed the<br /> physical Nyquist limit via pixel doubling before motion correction. We call this<br /> ‘post-acquisition super resolution’, or PASR. While this was originally developed<br /> for processing of giant virus datasets, where acquiring at high magnification<br /> is not always possible or desirable, it is also shown to work for smaller<br /> objects such as adeno-associated virus and apoferritin, both of which are high<br /> symmetry, and jack bean urease, with lower symmetry. PASR reduces the<br /> magnification required to achieve desired resolutions, which may increase<br /> collection efficiency. Further, PASR may be applied on top of acquisition-time<br /> super resolution data collection, allowing results to exceed not just the physical<br /> sampling limit, but also the super resolution sampling limit. However, to benefit<br /> from PASR, data must already reach the Nyquist limit.<br /> <br /> == Keywords ==<br /> <br /> == Links ==<br /> <br /> https://journals.iucr.org/m/issues/2026/05/00/rq5016/index.html<br /> <br /> == Related software ==<br /> <br /> == Related methods ==<br /> <br /> == Comments ==</div>

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