WikiSysop: Created page with "== Citation == Gauvin, C.C., Findlay, J.L., Hophan-Nichols, C. and Lawrence, C.M. 2026. A cost-effective 200 kV cryo-EM core facility for high resolution single particle analysis. Methods in Microscopy. (2026). == Abstract == We sought to establish a sustainable 200 kV cryo- EM core facility for the Northern Rocky Mountain region with high resolution single particle capabilities. With modest funding, relative to 300 kV instruemnts, from the NSF, theMurdock Charitable..."

2026-04-23T08:52:17Z

Created page with "== Citation == Gauvin, C.C., Findlay, J.L., Hophan-Nichols, C. and Lawrence, C.M. 2026. A cost-effective 200 kV cryo-EM core facility for high resolution single particle analysis. Methods in Microscopy. (2026). == Abstract == We sought to establish a sustainable 200 kV cryo- EM core facility for the Northern Rocky Mountain region with high resolution single particle capabilities. With modest funding, relative to 300 kV instruemnts, from the NSF, theMurdock Charitable..."

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== Citation ==

Gauvin, C.C., Findlay, J.L., Hophan-Nichols, C. and Lawrence, C.M. 2026. A cost-effective 200 kV cryo-EM core facility for high resolution single particle analysis. Methods in Microscopy. (2026).

== Abstract ==

We sought to establish a sustainable 200 kV cryo-
EM core facility for the Northern Rocky Mountain region
with high resolution single particle capabilities. With modest
funding, relative to 300 kV instruemnts, from the NSF,
theMurdock Charitable Trust and Montana State University,
we remodeled space and installed a 200 kV Talos Arctica
with aGatan K3 camera, along with supporting software and
computational resources. In addition, a refurbished T-12
Spirit was added to support negative stain work, and the
instrumentation was collectively organized as a university
core facility. The facility is now serving academic and industrial
users across the Northern Rocky Mountain region, and
beyond. We find that single particle data collection on low
symmetry particles greater than 100 kDa routinely gives
structures at better than 3 Å resolution, with several high
symmetry structures going past 2 Å. These efforts demonstrate
that Carnegie R1 - research intensive universities
are well served by 200 kV microscopes for single particle
work. Such microscopes approach the capabilities of more
expensive 300 kV instruments with their ability to produce
high resolution single particle structures, and can do so
at significant cost savings, contributing to sustainable core
facilities.

== Keywords ==

== Links ==

https://www.degruyterbrill.com/document/doi/10.1515/mim-2025-0039/html

== Related software ==

== Related methods ==

== Comments ==

2026Gauvin 200kV - Revision history