WikiSysop: Created page with "== Citation == Klaholz, B.P. 2026. A discussion of cryo-EM terminology as the outreach and number of PDB entries expand. IUCrJ. 13, 5 (2026). == Abstract == Cryo electron microscopy (cryo-EM) has made great advances in the last decade, progressively increasing its impact in structural biology as a key method to address molecular structures and mechanisms of various macromolecular complexes. Single-particle cryo-EM will soon equal the number of yearly entries in the Pr..."

2026-09-17T05:54:07Z

Created page with "== Citation == Klaholz, B.P. 2026. A discussion of cryo-EM terminology as the outreach and number of PDB entries expand. IUCrJ. 13, 5 (2026). == Abstract == Cryo electron microscopy (cryo-EM) has made great advances in the last decade, progressively increasing its impact in structural biology as a key method to address molecular structures and mechanisms of various macromolecular complexes. Single-particle cryo-EM will soon equal the number of yearly entries in the Pr..."

New page

== Citation ==

Klaholz, B.P. 2026. A discussion of cryo-EM terminology as the outreach and number of PDB entries expand. IUCrJ. 13, 5 (2026).

== Abstract ==

Cryo electron microscopy (cryo-EM) has made great advances in the last
decade, progressively increasing its impact in structural biology as a key method
to address molecular structures and mechanisms of various macromolecular
complexes. Single-particle cryo-EM will soon equal the number of yearly entries
in the Protein Data Bank from structures determined by X-ray crystallography.
This is largely thanks to improved cryo electron microscope instrumentation and
advanced image-processing tools and structure-sorting methods. As the role of
cryo-EM is expanding to an increasingly large community, including newcomers
and scientists joining from related fields, it is timely to revisit some fundamental
concepts and basics of single-particle cryo-EM and image processing as terminology
has become less well defined and, in some cases, confusing. Here we
summarize and define some typical terms important for understanding the
underlying physical concepts. These include ‘cryo-EM’, ‘cryo-ET’, ‘3D reconstruction’,
‘coarsening’, ‘contour level’ and others. We also discuss resolution
estimation and map deposition.

== Keywords ==

== Links ==

https://journals.iucr.org/m/issues/2026/05/00/hen5001/index.html

== Related software ==

== Related methods ==

== Comments ==

2026Klaholz Concepts - Revision history