WikiSysop: Created page with "== Citation == Mulvaney, T., Kryshtafovych, A. and Topf, M. 2026. Cryo-EM Analysis in CASP16. Proteins: Structure, Function, and Bioinformatics. (2026). == Abstract == Since CASP13, experimentalists have been encouraged to provide their cryo-EM data along with the derived atomic models to the CASP organizers to aid assessment. In CASP16, 38 cryo-EM datasets were provided for assessment, which represented most cryo-EM targets. The corresponding targets typically compri..."

2026-01-07T08:14:42Z

Created page with "== Citation == Mulvaney, T., Kryshtafovych, A. and Topf, M. 2026. Cryo-EM Analysis in CASP16. Proteins: Structure, Function, and Bioinformatics. (2026). == Abstract == Since CASP13, experimentalists have been encouraged to provide their cryo-EM data along with the derived atomic models to the CASP organizers to aid assessment. In CASP16, 38 cryo-EM datasets were provided for assessment, which represented most cryo-EM targets. The corresponding targets typically compri..."

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== Citation ==

Mulvaney, T., Kryshtafovych, A. and Topf, M. 2026. Cryo-EM Analysis in CASP16. Proteins: Structure, Function, and Bioinformatics. (2026).

== Abstract ==

Since CASP13, experimentalists have been encouraged to provide their cryo-EM
data along with the derived atomic models to
the CASP organizers to aid assessment. In CASP16, 38 cryo-EM
datasets were provided for assessment, which represented most
cryo-EM
targets. The corresponding targets typically comprised a single derived atomic structure; however, that model may be
only one of several valid conformations. Flexibility often manifests as low-resolution
regions in a cryo-EM
reconstruction, particularly
in RNA but often also in protein complexes. We show that local resolution in the reconstruction correlates well with the
root-mean-
square
fluctuations (RMSF) of residues of accurate CASP predictions. The correlation between the local resolution
and pLDDT was less clear, especially when mobile domains were present. When the resolution allowed, assessment of features
such as sidechains, using our variant of SMOC with local fragment alignment, indicated that even high-quality
predictions have
room for improvement; on the other hand, some predictions fitted the density better in specific regions, indicating modeling
discrepancies in the target. In one extreme case, a submitted target had regions of low-resolution
that limited unambiguous
model building. In such cases, part of the target structure is essentially a prediction itself, with implications for the assessment.
Experimental data remain essential for model-free
assessment of predictions and offer unique analyses such as comparisons to
local resolution and thus flexibility.

== Keywords ==

== Links ==

https://onlinelibrary.wiley.com/doi/full/10.1002/prot.70099

== Related software ==

== Related methods ==

== Comments ==

2026Mulvaney CASP16 - Revision history