https://3demmethods.i2pc.es/index.php?action=history&feed=atom&title=2026Premaraj_DualJet 2026-05-13T09:46:25Z Revision history for this page on the wiki MediaWiki 1.44.2 https://3demmethods.i2pc.es/index.php?title=2026Premaraj_DualJet&diff=5208&oldid=prev 2026-05-13T06:52:42Z

<p>Created page with &quot;== Citation == Premaraj, N., Huysmans, P., Ploum, M., Schijns, L., Peters, P.J., López-Iglesias, C., Ravelli, R.B. and Knoops, K. 2026. An experimental platform for exploring dual-jet vitrification mechanisms in cryo-EM sample preparation. Methods in Microscopy. 0 (2026). == Abstract == Jet vitrification harnesses the exceptionally high cooling potential of rapidly moving liquid cryogens to achieve ultrafast sample freezing with superior cooling efficiency. Building...&quot;</p> <p><b>New page</b></p><div>== Citation ==<br /> <br /> Premaraj, N., Huysmans, P., Ploum, M., Schijns, L., Peters, P.J., López-Iglesias, C., Ravelli, R.B. and Knoops, K. 2026. An experimental platform for exploring dual-jet vitrification mechanisms in cryo-EM sample preparation. Methods in Microscopy. 0 (2026).<br /> <br /> == Abstract ==<br /> <br /> Jet vitrification harnesses the exceptionally high cooling potential of rapidly moving liquid cryogens to achieve ultrafast sample freezing with superior cooling efficiency. Building on earlier work, this study provides a comprehensive investigation into the physical and technical principles underlying dual-jet vitrification. Developing ultra-rapid freezing methods that reliably produce vitreous cells, supports thicker samples, and integrate seamlessly into cryo-electron tomography (cryo-ET) workflows do represent important steps forward in the field. Here, we introduce an experimental platform designed to study the dynamics and efficacy of liquid-ethane jet vitrification at −180 °C. The system integrates multiple modules enabling controlled examination of the vitrification process at microsecond timescales, with a focus on quantifying temperature transitions, identifying optimal freezing parameters, and delineating the limitations of dual-jet configurations. The compact design allows operation within a standard biosafety cabinet and supports various sample holders compatible with biological specimens. Furthermore, we demonstrate how this setup can be directly incorporated into existing cryo-ET workflows using EM grids pre-mounted in AutoGrids. Overall, this work establishes a versatile platform for dissecting the mechanisms of ethane jet vitrification and provides methodological insights that support the preparation of thicker, well-preserved biological samples for in situ structural analysis.<br /> <br /> == Keywords ==<br /> <br /> == Links ==<br /> <br /> https://www.degruyterbrill.com/document/doi/10.1515/mim-2025-0035/html<br /> <br /> == Related software ==<br /> <br /> == Related methods ==<br /> <br /> == Comments ==</div>

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