1994Penczek Real: Difference between revisions
No edit summary |
No edit summary |
||
| Line 14: | Line 14: | ||
== Keywords == | == Keywords == | ||
3D Angular assignment | |||
== Links == | == Links == | ||
| Line 21: | Line 21: | ||
== Related software == | == Related software == | ||
Xmipp: http://xmipp.cnb.uam.es/twiki/bin/view/Xmipp/Projection_matching | |||
== Related methods == | == Related methods == | ||
== Comments == | == Comments == | ||
Latest revision as of 12:06, 6 April 2009
Citation
Penczek PA, Grassucci RA, Frank J (1994) The ribosome at improved resolution: new techniques for merging and orientation refinement in 3D cryo-electron microscopy of biological particles. Ultramicroscopy 53:251–270
Abstract
Cryo-electron microscopy of single biological particles opens up new possibilities for structure analysis: the particle can be reconstructed in its native shape and internal features are preserved. To take advantage of these possibilities we have developed new methods of data collection and image processing and we have applied them to the 70S Escherichia coli ribosome. A method of orientation search is proposed, which makes it possible to relate random-conical data sets to one another even if they are collected from low-tilt micrographs. A technique for 3D alignment of projections is described and applied to the single-micrograph 3D reconstruction.
Keywords
3D Angular assignment
Links
Article http://www.ncbi.nlm.nih.gov/pubmed/8160308
Related software
Xmipp: http://xmipp.cnb.uam.es/twiki/bin/view/Xmipp/Projection_matching