2004Sorzano IDR: Difference between revisions
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Sorzano, C. O. S.; Marabini, R.; Herman, G. T.; Censor, Y. & Carazo, J. M. Transfer function restoration in 3D electron microscopy via iterative data refinement Physics in Medicine & Biology, 2004, 49, 509-522 | Sorzano, C. O. S.; Marabini, R.; Herman, G. T.; Censor, Y. & Carazo, J. M. Transfer function restoration in 3D electron microscopy via iterative data refinement Physics in Medicine & Biology, 2004, 49, 509-522 | ||
[http://scholar.google.com/scholar?hl=en&lr=&newwindow=1&cites=6549846159713804781 Cited by] | [http://scholar.google.com/scholar?hl=en&lr=&newwindow=1&cites=6549846159713804781 Cited by] | ||
Latest revision as of 12:17, 1 April 2009
Citation
Sorzano, C. O. S.; Marabini, R.; Herman, G. T.; Censor, Y. & Carazo, J. M. Transfer function restoration in 3D electron microscopy via iterative data refinement Physics in Medicine & Biology, 2004, 49, 509-522
Abstract
Three-dimensional electron microscopy (3D-EM) is a powerful tool for visualizing complex biological systems. As with any other imaging device, the electron microscope introduces a transfer function (called in this field the contrast transfer function, CTF) into the image acquisition process that modulates the various frequencies of the signal. Thus, the 3D reconstructions performed with these CTF-affected projections are also affected by an implicit 3D transfer function. For high-resolution electron microscopy, the effect of the CTF is quite dramatic and limits severely the achievable resolution. In this work we make use of the iterative data refinement (IDR) technique to ameliorate the effect of the CTF. It is demonstrated that the approach can be successfully applied to noisy data.
Keywords
CTF restoration
Links
Article http://math.haifa.ac.il/yair/PMBfinalaccepted041203.pdf
Related software
Xmipp: http://xmipp.cnb.csic.es/twiki/bin/view/Xmipp/IDR_ART