2016Zhang DNANG: Difference between revisions
Garygangren (talk | contribs) (Created page with "== Citation == Molecular Organization of the Early Stages of Nucleosome Phase Separation Visualized by Cryo-Electron Tomography, Meng Zhang, César Díaz-Celis, Bibiana Onoa, Cristhian Cañari-Chumpitaz, Katherinne I. Requejo, Jianfang Liu, Michael Vien, Eva Nogales, Gang Ren, and Carlos Bustamante, Molecular Cell, (2022), 18;82(16):3000-3014.e9; PMID: 35907400 DOI: 10.1016/j.molcel.2022.06.032 == Abstract == It has been proposed that the intrinsic property of nucleosom...") |
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== Citation == | == Citation == | ||
3D Structural Dynamics and Fluctuations of DNA-Nanogold Conjugates by Individual-Particle Electron Tomography, Lei Zhang, Dongsheng Lei, Jessica M. Smith, Huimin Tong, Xing Zhang, Zhuoyang Lu, Paul Alivisatos and Gang Ren, Nature Communications, (2016), 7:11083. doi: 10.1038 | |||
== Abstract == | == Abstract == | ||
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== Keywords == | == Keywords == | ||
Single molecule structure, individual-particle electron tomography, IPET, DNA nanogold conjugate | |||
== Paper links == | == Paper links == | ||
https:// | https://www.nature.com/articles/ncomms11083 | ||
== Related video == | == Related video == | ||
https://www. | https://www.youtube.com/watch?v=lQrbmg9ry90 | ||
== Comments == | == Comments == | ||
Latest revision as of 09:28, 12 November 2024
Citation
3D Structural Dynamics and Fluctuations of DNA-Nanogold Conjugates by Individual-Particle Electron Tomography, Lei Zhang, Dongsheng Lei, Jessica M. Smith, Huimin Tong, Xing Zhang, Zhuoyang Lu, Paul Alivisatos and Gang Ren, Nature Communications, (2016), 7:11083. doi: 10.1038
Abstract
It has been proposed that the intrinsic property of nucleosome arrays to undergo liquid-liquid phase separation (LLPS) in vitro is responsible for chromatin domain organization in vivo. However, understanding nucleosomal LLPS has been hindered by the challenge to characterize the structure of the resulting heterogeneous condensates. We used cryo-electron tomography and deep-learning-based 3D reconstruction/segmentation to determine the molecular organization of condensates at various stages of LLPS. We show that nucleosomal LLPS involves a two-step process: a spinodal decomposition process yielding irregular condensates, followed by their unfavorable conversion into more compact, spherical nuclei that grow into larger spherical aggregates through accretion of spinodal materials or by fusion with other spherical condensates. Histone H1 catalyzes more than 10-fold the spinodal-to-spherical conversion. We propose that this transition involves exposure of nucleosome hydrophobic surfaces causing modified inter-nucleosome interactions. These results suggest a physical mechanism by which chromatin may transition from interphase to metaphase structures.
Keywords
Single molecule structure, individual-particle electron tomography, IPET, DNA nanogold conjugate
Paper links
https://www.nature.com/articles/ncomms11083
Related video
https://www.youtube.com/watch?v=lQrbmg9ry90