2013Shigematsu H Noisemodels

Citation

Shigematsu H, Sigworth FJ.Noise models and cryo-EM drift correction with a direct-electron camera.U ltramicroscopy. 2013 Aug;131:61-9.

Abstract

The apparent handedness of an EM-tomography reconstruction depends on a number of conventions andc an be confused in many ways. As the number of different hardware and software combinations being used for electron tomography continue to climb, and the reconstructions being produced reach higherand higher resolutions, the need to verify the hand of the results has increased. Here we enumerate various steps in a typical tomography experiment that affect handedness and show that DNA origami gold nanoparticle helices can be used as convenient and fail-safe handedness standards.

Keywords

On the use of DNA origami gold particles as internal handedness starndar

Links

http://www.ncbi.nlm.nih.gov/pubmed/23748163