2021Sorzano Uneven
Citation
Sorzano, C.; Semchonok, D.; Lin, S.; Lo, Y.; Vilas, J.; Jiménez-Moreno, A.; Gragera, M.; Vacca, S.; Maluenda, D.; Mart\inez, M.; Ramírez-Aportela, E.; Melero, R.; Cuervo, A.; Conesa, J. J.; Conesa, P.; Losana, P.; del Caño, L.; Jiménez de la Morena, J.; Fonseca, Y. C.; Sánchez-García, R.; Strelak, D.; Fernández-Giménez, E.; de Isidro, F.; Herreros, D.; Kastritis, P.; Marabini, R.; Bruce, B. D.; Carazo, J. M. Algorithmic robustness to preferred orientations in Single Particle Analysis by CryoEM. J. Structural Biology, 2021, 213, 107695
Abstract
The presence of preferred orientations in single particle analysis (SPA) by cryo-Electron Microscopy (cryoEM) is currently one of the hurdles preventing many structural analyses from yielding high-resolution structures. Although the existence of preferred orientations is mostly related to the grid preparation, in this technical note, we show that some image processing algorithms used for angular assignment and three-dimensional (3D) reconstruction are more robust than others to these detrimental conditions. We exemplify this argument with three different data sets in which the presence of preferred orientations hindered achieving a 3D reconstruction without artifacts or, even worse, a 3D reconstruction could never be achieved.
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Links
https://www.sciencedirect.com/science/article/pii/S1047847720302689