Taylor, K. A. & Glaeser, R. M. Electron diffraction of frozen, hydrated protein crystals. Science, 1974 , 186 , 1036-1037
High-resolution electron diffraction patterns have been obtained from frozen, hydrated catalase crystals to demonstrate the feasibility of using a frozen specimen hydration technique. The use of frozen specimens to maintain the hydration of complex biological structures has certain advantages over previously developed liquid hydration techniques.