Sorzano, C. O. S.; de la Fraga, L. G.; Clackdoyle, R. & Carazo, J. M. Normalizing projection images: A study of image normalizing procedures for single particle three-dimensional electron microscopy Ultramicroscopy, 2004, 101, 129-138
In the process ofthree-dimensional reconstruction ofsingle particle biological macromolecules several hundreds, or thousands, ofprojection images are taken from tens or hundreds of independently digitized micrographs. These different micrographs show differences in the background grey level and particle contrast and, therefore, have to be normalized by scaling their pixel values before entering the reconstruction process. In this work several normalization procedures are studied using a statistical comparison framework. We finally show that the use of the different normalization methods affects the reconstruction quality, providing guidance on the choice of normalization procedures.
Image normalization, image formation model