Nickell S, Kofler C, Leis AP, Baumeister W (2006) A visual approach to proteomics. Nat Rev Mol Cell Biol 7:225–230
Cryo-electron tomography is an emerging imaging technique that has unique potential for molecular cell biology. At the present resolution of 4-5 nm, large supramolecular structures can be studied in unperturbed cellular environments and, in the future, it will become possible to map molecular landscapes inside cells in a more comprehensive manner. 'Visual proteomics' aims to complement and extend mass-spectrometry-based inventories, and to provide a quantitative description of the macromolecular interactions that underlie cellular functions.
Review, visual proteomics, single particles, localization, electron tomography