Grigorieff N. (2007) FREALIGN: High-resolution refinement of single particle structures. J Struct Biol 157(1)117–125
The refinement of three-dimensional reconstructions and correction for the contrast transfer function of the microscope are important steps in the determination of macromolecular structures by single particle electron microscopy. The algorithms implemented in the com- puter program FREALIGN are optimized to perform these tasks efficiently. A general overview and details on how to use FREALIGN are provided. The program is free and available for download on the author’s web page.
Image processing, Electron microscopy, Protein structure, FREALIGN
Related software: http://grigoriefflab.janelia.org/software
| Paper | 2013Lyumkis D_FREALIGN | Likelihood-based classification of cryo-EM images using FREALIGN.