Langlois, R.; Pallesen, J.; Ash, J. T.; Nam Ho, D.; Rubinstein, J. L. & Frank, J. Automated particle picking for low-contrast macromolecules in cryo-electron microscopy. J Struct Biol, 2014, 186, 1-7
Cryo-electron microscopy is an increasingly popular tool for studying the structure and dynamics of biological macromolecules at high resolution. A crucial step in automating single-particle reconstruction of a biological sample is the selection of particle images from a micrograph. We present a novel algorithm for selecting particle images in low-contrast conditions; it proves more effective than the human eye on close-to-focus micrographs, yielding improved or comparable resolution in reconstructions of two macromolecular complexes.