Barad, B. A.; Echols, N.; Wang, R. Y.-R.; Cheng, Y.; DiMaio, F.; Adams, P. D. & Fraser, J. S. EMRinger: side chain-directed model and map validation for 3D cryo-electron microscopy. Nat Methods, 2015, 12, 943-946
Advances in high-resolution cryo-electron microscopy (cryo-EM) require the development of validation metrics to independently assess map quality and model geometry. We report EMRinger, a tool that assesses the precise fitting of an atomic model into the map during refinement and shows how radiation damage alters scattering from negatively charged amino acids. EMRinger (https://github.com/fraser-lab/EMRinger) will be useful for monitoring progress in resolving and modeling high-resolution features in cryo-EM.