Difference between revisions of "2016Glaeser Behaviour"
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Latest revision as of 12:19, 1 February 2018
Citation
Glaeser, R. M. Specimen behavior in the electron beam. Methods in Enzymology. The Resolution Revolution: Recent Advances In cryoEM, 2016, 19-50
Abstract
It has long been known that cryo-EM specimens are severely damaged by a level of electron exposure that is much lower than what is needed to obtain high-resolution images from single macromolecules. Perhaps less well appreciated in the cryo-EM literature, the vitreous ice in which samples are suspended is equally sensitivity to radiation damage. This chapter provides a review of several fundamental topics such as inelastic scattering of electrons, radiation chemistry, and radiation biology, which-together-can help one to understand why radiation damage occurs so "easily." This chapter also addresses the issue of beam-induced motion that occurs at even lower levels of electron exposure. While specimen charging may be a contributor to this motion, it is argued that both radiation-induced relief of preexisting stress and damage-induced generation of additional stress may be the dominant causes of radiation-induced movement.
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Links
https://www.ncbi.nlm.nih.gov/pubmed/27572722