Difference between revisions of "2018Sieben Multicolor"
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Latest revision as of 07:43, 31 January 2019
Citation
Sieben, C.; Banterle, N.; Douglass, K. M.; Gönczy, P. & Manley, S. Multicolor single-particle reconstruction of protein complexes. Nature methods, 2018, 15, 777-780
Abstract
Single-particle reconstruction (SPR) from electron microscopy (EM) images is widely used in structural biology, but it lacks direct information on protein identity. To address this limitation, we developed a computational and analytical framework that reconstructs and coaligns multiple proteins from 2D super-resolution fluorescence images. To demonstrate our method, we generated multicolor 3D reconstructions of several proteins within the human centriole, which revealed their relative locations, dimensions and orientations.
Keywords
Links
https://www.nature.com/articles/s41592-018-0140-x.pdf
