Difference between revisions of "2021Jagota GoldNanoparticles"
(Created page with "== Citation == Jagota, M.; Townshend, R. J. L.; Kang, L.-W.; Bushnell, D. A.; Dror, R. O.; Kornberg, R. D. & Azubel, M. Gold nanoparticles and tilt pairs to assess protei...") |
(No difference)
|
Latest revision as of 09:40, 16 June 2021
Citation
Jagota, M.; Townshend, R. J. L.; Kang, L.-W.; Bushnell, D. A.; Dror, R. O.; Kornberg, R. D. & Azubel, M. Gold nanoparticles and tilt pairs to assess protein flexibility by cryo-electron microscopy. Ultramicroscopy, 2021, 227, 113302
Abstract
A computational method was developed to recover the three-dimensional coordinates of gold nanoparticles specifically attached to a protein complex from tilt-pair images collected by electron microscopy. The program was tested on a simulated dataset and applied to a real dataset comprising tilt-pair images recorded by cryo electron microscopy of RNA polymerase II in a complex with four gold-labeled single-chain antibody fragments. The positions of the gold nanoparticles were determined, and comparison of the coordinates among the tetrameric particles revealed the range of motion within the protein complexes.
Keywords
Links
https://www.sciencedirect.com/science/article/pii/S0304399121000899