2000Tao Review

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Tao Y, Zhang W (2000) Recent developments in cryo-electron microscopy reconstruction of single particles. Curr Opin Struct Biol 10:616–622

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Cryo-electron microscopy and single-particle 3D image reconstruction techniques have been used to examine a broad spectrum of samples ranging from 500 kDa protein complexes to large subcellular organelles. The attainable resolution has improved rapidly over the past few years. Structures of both symmetric and asymmetric assemblies at approximately 7.5 A have been reported. Together with X-ray crystallography, three-dimensional cryo-electron microscopy reconstruction has provided important insights into the function of many biological systems in their native biochemical contexts.


Review, single particles


Article http://www.ncbi.nlm.nih.gov/pubmed/11042462

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