2016Grigorieff Frealign

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Grigorieff, N. Methods in Enzymology. The Resolution Revolution: Recent Advances In cryoEM Frealign: an exploratory tool for single-particle cryo-EM Academic Press, 2016, 191-226


Frealign is a software tool designed to process electron microscope images of single molecules and complexes to obtain reconstructions at the highest possible resolution. It provides a number of refinement parameters and options that allow users to tune their refinement to achieve specific goals, such as masking to classify selected regions within a particle, control over the refinement of specific alignment parameters to accommodate various data collection schemes, refinement of pseudosymmetric particles, and generation of initial maps. This chapter provides a general overview of Frealign functions and a more detailed guide to using Frealign in typical scenarios.




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