2016Koeck ADF

Citation

Koeck, P. J. B. Annular dark field transmission electron microscopy for protein structure determination. Ultramicroscopy, 2016, 161, 98-104

Abstract

Recently annular dark field (ADF) transmission electron microscopy (TEM) has been advocated as a means of recording images of biological specimens with better signal to noise ratio (SNR) than regular bright field images. I investigate whether and how such images could be used to determine the three-dimensional structure of proteins given that an ADF aperture with a suitable pass-band can be manufactured and used in practice. I develop an approximate theory of ADF-TEM image formation for weak amplitude and phase objects and test this theory using computer simulations. I also test whether these simulated images can be used to calculate a three-dimensional model of the protein using standard software and discuss problems and possible ways to overcome these.

Links

http://www.ncbi.nlm.nih.gov/pubmed/26656466