2017Zheng MotionCorr2

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Zheng, S. Q.; Palovcak, E.; Armache, J.-P.; Verba, K. A.; Cheng, Y. & Agard, D. A. MotionCor2: anisotropic correction of beam-induced motion for improved cryo-electron microscopy. Nature methods, 2017, 14, 331-332


In recent years single-particle cryo-electron microscopy (cryo-EM) has enabled groundbreaking advancements in obtaining atomic-resolution structures of macromolecules1, 2. Central to this success has been the broad application of direct electron detector cameras with high output frame rates, which has enabled the recording of images of frozen hydrated biological samples as dose-fractionated stacks of subframes (movies). Beam-induced sample motion that blurs the captured images can then be corrected by registering identical features in the subframes, followed by summing the registered subframes to produce a motion-corrected image3. Here we describe MotionCor2, a software tool for anisotropic correction of beam-induced motion




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