2017Frank Review: Difference between revisions
Created page with "== Citation == Frank, J. Advances in the field of single-particle cryo-electron microscopy over the last decade. Nature protocols, 2017, 12, 209-212 == Abstract == In singl..." |
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Latest revision as of 13:39, 12 January 2017
Citation
Frank, J. Advances in the field of single-particle cryo-electron microscopy over the last decade. Nature protocols, 2017, 12, 209-212
Abstract
In single-particle cryo-electron microscopy (cryo-EM), molecules suspended in a thin aqueous layer are rapidly frozen and imaged at cryogenic temperature in the transmission electron microscope. From the random projection views, a three-dimensional image is reconstructed, enabling the structure of the molecule to be obtained. In this article I discuss technological progress over the past decade, which has, in my own field of study, culminated in the determination of ribosome structure at 2.5-Å resolution. I also discuss likely future improvements in methodology.
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Links
http://www.nature.com/nprot/journal/v12/n2/full/nprot.2017.004.html